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Y-17
Y-17
規(guī)格:
貨期:
編號:B165981
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 Y-17
商品貨號 B165981
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Strain

Strain:? BALB/c (B cell); BALB/c (myeloma)

Applications
The antibody reacts with a conformational or combinatorial determinant on Ea:Eb complexes (Ae:Ea) of the b, k, r, s and v haplotypes.
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused with Sp2/0 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against I-E; cytotoxic
Cellular Products
immunoglobulin; monoclonal antibody; against I-E; cytotoxic
Comments
Animals were immunized with Con A stimulated spleen cells from B10.A(5R) mice.
Spleen cells were fused with Sp2/0 myeloma cells.
The antibody reacts with a conformational or combinatorial determinant on Ea:Eb complexes (Ae:Ea) of the b, k, r, s and v haplotypes.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001.To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 5%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
Freeze medium: complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Isotype IgG2b
Name of Depositor C Janeway
Deposited As mouse (B cell); mouse (myeloma)
References

Lerner EA, et al. Monoclonal antibody against an Ir gene product. J. Exp. Med. 152: 1085-1101, 1980. PubMed: 6158546

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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