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WEHI 7.1
WEHI 7.1
規(guī)格:
貨期:
編號:B165938
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 WEHI 7.1
商品貨號 B165938
Organism Mus musculus, mouse
Tissue thymus
Cell Type T lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease lymphoma
Strain BALB/c
Applications
Derived from the WEHI 7 thymoma induced by X-irradiation of a BALB/c mouse.
Sensitive to both cortisol and dexamethasone.
Tested and found negative for ectromelia virus (mousepox).
Derivation
Derived from the WEHI 7 thymoma induced by X-irradiation of a BALB/c mouse.
Antigen Expression
H-2d; Thy-1.2
Genes Expressed
H-2d; Thy-1.2
Comments
Derived from the WEHI 7 thymoma induced by X-irradiation of a BALB/c mouse.
Sensitive to both cortisol and dexamethasone.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 2- 3 x 10 exp5 viable cells/ml.
A saturation density of 1-2 X 10 exp6 is obtainable.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Name of Depositor AW Harris
Deposited As Mus musculus
References

Pfahl M, et al. General features of steroid resistance in lymphoid cell lines. Mol. Cell. Endocrinol. 10: 193-207, 1978. PubMed: 566227

Harris AW, et al. Differentiated functions expressed by cultured mouse lymphoma cells. II. theta Antigen, surface immunoglobulin and a receptor for antibody in cells of a thymoma cell line. J. Immunol. 110: 431-438, 1973. PubMed: 4630643

Bourgeois S, Newby RF. Diploid and haploid states of the glucocorticoid receptor gene of mouse lymphoid cell lines. Cell 11: 423-460, 1977. PubMed: 560913

Pfahl M, et al. Interaction of glucocorticoid receptors from lymphoid cell lines with their nuclear acceptor sites. Mol. Cell. Endocrinol. 10: 175-191, 1978. PubMed: 26620

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