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WEHI 22.1
WEHI 22.1
規(guī)格:
貨期:
編號:B165937
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產品名稱 WEHI 22.1
商品貨號 B165937
Organism Mus musculus, mouse
Tissue thymus
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease lymphoma
Strain BALB/c
Applications
The line was derived from a thymoma induced by X-irradiation.
They are sensitive to 100 nM cortisol.
Tested and found negative for ectromelia virus (mousepox).
Derivation
The line was derived from a thymoma induced by X-irradiation.
Antigen Expression
Thy-1.2
Genes Expressed
immunoglobulin; monoclonal antibody,Thy-1.2
Cellular Products
immunoglobulin; monoclonal antibody
Comments
The line was derived from a thymoma induced by X-irradiation.
The cells have characteristics of both T and B cells and form rosettes with sheep erythrocytes.
They are sensitive to 100 nM cortisol.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype IgM (surface)
Name of Depositor AW Harris
Deposited As Mus musculus
References

Harris AW, et al. Differentiated functions expressed by cultured mouse lymphoma cells. II. theta Antigen, surface immunoglobulin and a receptor for antibody in cells of a thymoma cell line. J. Immunol. 110: 431-438, 1973. PubMed: 4630643

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