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UCD/AB 6.01
UCD/AB 6.01
規(guī)格:
貨期:
編號:B165908
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 UCD/AB 6.01
商品貨號 B165908
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease Cancer
Applications
The antibody is specific for type 8 cytokeratin (52000 dalton).
It cross-reacts somewhat with types 18 and 19 cytokeratins (46000 dalton and 40000 dalton, respectively).
The antibody can be used to detect cytokeratin released by neoplastic cells into serum.
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against a human mammary carcinoma cell line (MCF7, see ATCC HTB-22)
Cellular Products
immunoglobulin; monoclonal antibody; against a human mammary carcinoma cell line (MCF7, see ATCC HTB-22)
Comments
Animals were immunized with the MCF7 human mammary cancer cell line.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody is specific for type 8 cytokeratin (52000 dalton).
It cross-reacts somewhat with types 18 and 19 cytokeratins (46000 dalton and 40000 dalton, respectively).
The antibody can be used to detect cytokeratin released by neoplastic cells into serum.
Complete Growth Medium

Culture medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain

1.15 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium

pyruvate, 90%; fetal bovine serum, 10%.

Reference: U.S. Pat. 4,775,620.

Deposited on behalf of:? University of California

???????????????????????? Davis, CA

Cell lines and hybridomas deposited for patent purposes are not always screened

for contamination, antibody production or characterized by the ATCC. Release of

a culture, the use of which may be claimed in a patent, from the ATCC during

the effective term of any such patent is not meant to carry with it, and does

not grant any license, express or implied, under any patent, or the right to

use a culture in any process described in a patent.

The above culture was deposited in the ATCC in connection with a patent

application.

Copies of U.S. Patents may be obtained from the Commissioner of Patents,

U.S. Patent and Trademark Office, Box 9, Washington, D.C. 20231. A coupon

is required which is purchased through the office above, and on which the

information is submitted to request the patent.

???????????????????????????????????????????????????????????????????????? (1/98)

.CCLSFTY.FACTSHTS.VXL

Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
culture medium, 90%; DMSO, 10%
Name of Depositor Regents of the University of California
Deposited As mouse (B cell); mouse (myeloma)
U.S. Patent Number
References

Cardiff RD, et al. Cytokeratin tumor markers and assays for their detection. US Patent 4,775,620 dated Oct 4 1988

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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