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U266B1 [U266]
U266B1 [U266]
規(guī)格:
貨期:
編號:B165898
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 U266B1 [U266]
商品貨號 B165898
Organism Homo sapiens, human
Tissue
peripheral blood
Cell Type B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease myeloma; plasmacytoma
Age 53 years adult
Gender male
Applications
This cell line is a suitable transfection host.
Images Cell Micrograph of ATCC TIB-196, U266B1
Clinical Data
male
Genes Expressed
immunoglobulin; monoclonal antibody; interleukin 6 (interleukin-6, IL-6)
Cellular Products
immunoglobulin; monoclonal antibody; interleukin 6 (interleukin-6, IL-6)
Comments
U266 cells have been reported to produce human IL-6.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 15%.
Subculturing
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 3 x 105 cells/mL and maintain between 1 x 105 and 1 x 106 cells/mL. Corning® T-75 flasks (catalog #431464) are recommended for subculturing this product.
Medium Renewal: Every 2 to 3 days
Cryopreservation
culture medium 95%; DMSO, 5%
STR Profile
Amelogenin: X,Y
CSF1PO: 12,13
D13S317: 12
D16S539: 10
D5S818: 11,12
D7S820: 11,12
THO1: 5,7
TPOX: 8
vWA: 17
Isotype IgE; lambda light chain
Name of Depositor NK Nilsson
Deposited As Homo sapiens
References

Nilsson K, et al. Established immunoglobulin producing myeloma (IgE) and lymphoblastoid (IgG) cell lines from an IgE myeloma patient. Clin. Exp. Immunol. 7: 477-489, 1970. PubMed: 4097745

Kawano M, et al. Autocrine generation and requirement of BSF-2/IL-6 for human multiple myelomas. Nature 332: 83-85, 1988. PubMed: 3258060

Kolanus W, et al. alphaLbeta2 integrin/LFA-1 binding to ICAM-1 induced by cytohesin-1 a cytoplasmic regulatory molecule. Cell 86: 233-242, 1996. PubMed: 8706128

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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