91精品国产综合成人,国产精品午夜自在在线精品,国内精品久久久久影院老司,国内精品久久久久久久久野战,国产精品美女久久福利网站,亚洲国产一成人久久精品,国产精品久久久久久一区二区三区

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購(gòu)物車 1 種商品 - 共0元
當(dāng)前位置: 首頁(yè) > ATCC代理 > TRA-171
最近瀏覽歷史
聯(lián)系我們
  • 0574-87157013
  • [email protected]
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號(hào)
  • 創(chuàng)e慧谷42號(hào)樓B幢401室
TRA-171
TRA-171
規(guī)格:
貨期:
編號(hào):B165878
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 TRA-171
商品貨號(hào) B165878
Organism Toxorhynchites amboinensis, mosquito
Tissue larva, whole
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age larva
Storage Conditions liquid nitrogen vapor phase
Complete Growth Medium MThe base medium for this cell line is a 1:1 mixture of MM (Mitsuhashi and Maramorosch) and VP12 Medium (J. Med. Entomol. 6:432-439, 1969. PubMed: 5360492) To make the complete growth medium, add the following components to the base medium:
  • heat-inactivated fetal bovine serum to a final concentration of 10%.
  • Subculturing Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
    1. Remove and discard culture medium.
    2. Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum, which contains trypsin inhibitor.
    3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope at room temperature until cell layer is dispersed (usually within 5 to 10 minutes).
    4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
    5. Add appropriate aliquots of the cell suspension to new culture vessels.
    6. Incubate cultures at 28°C without a mixture of CO2 in air.

    Subcultivation Ratio: 1:2
    Medium Renewal: Every 2 to 3 days

    Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 13 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Wiley-Liss, N.Y., 2005.

    Cryopreservation

    Complete growth medium described above supplemented with 5% (v/v) DMSO.  Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

    Culture Conditions
    Temperature: 28°C
    Atmosphere: Air, 100%
    Population Doubling Time 28 hrs
    Name of Depositor G Kuno
    Deposited As Toxorhynchites amboinensis
    References

    Kuno G. New cell line. A continuous cell line of a nonhematophagous mosquito, Toxorhynchites amboinensis. In Vitro 16: 915-917, 1980.

    Varma MG, Pudney M. The growth and serial passage of cell lines from Aedes aegypti (L.) larvae in different media. J. Med. Entomol. 6: 432-439, 1969. PubMed: 5360492

    Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

    Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

    Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

    梅經(jīng)理 17280875617 1438578920
    胡經(jīng)理 13345964880 2438244627
    周經(jīng)理 17757487661 1296385441
    于經(jīng)理 18067160830 2088210172
    沈經(jīng)理 19548299266 2662369050
    李經(jīng)理 13626845108 972239479
    寻乌县| 绵阳市| 遂宁市| 桃园县| 大方县| 竹山县| 长宁区| 嘉峪关市| 鄂伦春自治旗| 应城市| 龙口市| 平度市| 贞丰县| 梁平县| 永寿县| 鄱阳县| 包头市| 嘉兴市| 石楼县| 永寿县| 武隆县| 巫山县| 宜昌市| 长垣县| 莱阳市| 囊谦县| 鄂托克旗| 电白县| 瑞金市| 阳信县| 城步| 海盐县| 蛟河市| 东城区| 汕尾市| 恩平市| 财经| 平阳县| 任丘市| 台东市| 江门市|