91精品国产综合成人,国产精品午夜自在在线精品,国内精品久久久久影院老司,国内精品久久久久久久久野战,国产精品美女久久福利网站,亚洲国产一成人久久精品,国产精品久久久久久一区二区三区

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當前位置: 首頁 > ATCC代理 > TD.1
▍最近瀏覽歷史
▍聯(lián)系我們
  • 0574-87157013
  • [email protected]
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號
  • 創(chuàng)e慧谷42號樓B幢401室
TD.1
TD.1
規(guī)格:
貨期:
編號:B165832
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 TD.1
商品貨號 B165832
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications
The antibody is useful for blotting of clathrin heavy chains from a variety of species.
Storage Conditions liquid nitrogen vapor phase
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human clathrin heavy chain
Cellular Products
immunoglobulin; monoclonal antibody; against human clathrin heavy chain
Comments
Animals were immunized with the globular N-terminal domain of clathrin heavy chain.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody is useful for blotting of clathrin heavy chains from a variety of species. It does not react with native clathrin in immunofluorescence or immunoprecipitation assays.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 15%.
Subculturing Cultures can be established by addition of fresh medium or replacement of medium. Alternatively cultures can be established by centrifugation with subsequent resuspension at 2 x 105 viable cells/mL. Maintain cell density between 105 and 106 viable cells/mL.
Medium Renewal: Two to three times weekly
Cryopreservation

Complete growth medium described above supplemented with 5% (v/v) DMSO.  Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
Isotype IgG1
Name of Depositor FM Brodsky
Deposited As mouse (B cell); mouse (myeloma)
References

Nathke IS, et al. Folding and trimerization of clathrin subunits at the triskelion hub. Cell 68: 899-910, 1992. PubMed: 1547490

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
克什克腾旗| 邵武市| 曲水县| 白朗县| 防城港市| 东阿县| 中宁县| 双峰县| 沧州市| 三穗县| 富裕县| 上犹县| 霸州市| 沂源县| 大丰市| 共和县| 山西省| 杨浦区| 绍兴市| 眉山市| 临泽县| 多伦县| 若羌县| 开平市| 金塔县| 丰顺县| 海南省| 柏乡县| 和田市| 凤凰县| 兴化市| 康定县| 宾川县| 资源县| 沁水县| 福州市| 双城市| 辽宁省| 科尔| 景泰县| 红安县|