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S49.1TB.2
S49.1TB.2
規(guī)格:
貨期:
編號:B165653
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 S49.1TB.2
商品貨號 B165653
Organism Mus musculus, mouse
Cell Type T lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease lymphoma
Strain BALB/c
Applications
This line was derived from S49.1 (ATCC TIB-28) by selecting for resistance to 10 mM thymidine and 0.1 mM 5-bromo-2'-deoxyuridine.
Like the parent line, the cells are sensitive to 0.001 mM cortisol (hydrocortisone), 5 mcg/ml phytohemagglutinin and 0.01 mM 6-thioguanine.
Tested and found negative for ectromelia virus (mousepox).
Derivation
This line was derived from S49.1 (ATCC TIB-28) by selecting for resistance to 10 mM thymidine and 0.1 mM 5-bromo-2'-deoxyuridine.
Antigen Expression
H-2d; Thy-1.2; TL
Genes Expressed
H-2d; Thy-1.2; TL
Comments
This line was derived from S49.1 (ATCC TIB-28) by selecting for resistance to 10 mM thymidine and 0.1 mM 5-bromo-2'-deoxyuridine.
Like the parent line, the cells are sensitive to 0.001 mM cortisol (hydrocortisone), 5 mcg/ml phytohemagglutinin and 0.01 mM 6-thioguanine.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium Dulbecco's modified Eagle's medium, 90%; horse serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Name of Depositor A Harris
Deposited As Mus musculus
References

Ralph P, et al. Independence of theta and TL surface antigens and killing by thymidine, cortisol, phytohemagglutinin, and cyclic AMP in a murine lymphoma. Biochem. Biophys. Res. Commun. 55: 1085-1091, 1973. PubMed: 4358928

Horibata K, Harris AW. Mouse myelomas and lymphomas in culture. Exp. Cell Res. 60: 61-77, 1970. PubMed: 5439579

Schiller JG, et al. Glucocorticoid receptors in lymphoma cells in culture: relationship to glucocorticoid killing activity. Science 171: 189-191, 1971. PubMed: 4395230

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