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S49.1G.3 PHA.100/0 (replaces S49.1G3 PHA.12P)
S49.1G.3 PHA.100/0 (replaces S49.1G3 PHA.12P)
規(guī)格:
貨期:
編號(hào):B165651
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 S49.1G.3 PHA.100/0 (replaces S49.1G3 PHA.12P)
商品貨號(hào) B165651
Organism Mus musculus, mouse
Cell Type T lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease lymphoma
Strain BALB/c
Applications
The cells are also resistant to 6-thioguanine and sensitive to thymidine, and hydrocortisone.
Tested and found negative for ectromelia virus (mousepox).
Antigen Expression
H-2d; Thy-1.2; TL
Genes Expressed
H-2d; Thy-1.2; TL
Comments
This variant of S49.1G.3 (ATCC TIB-34) was selected for resistance to 5 mcg/ml phytohemagglutinin.
The cells are also resistant to 6-thioguanine and sensitive to thymidine, and hydrocortisone.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Name of Depositor P Ralph
Deposited As Mus musculus
References

Ralph P, et al. Independence of theta and TL surface antigens and killing by thymidine, cortisol, phytohemagglutinin, and cyclic AMP in a murine lymphoma. Biochem. Biophys. Res. Commun. 55: 1085-1091, 1973. PubMed: 4358928

Horibata K, Harris AW. Mouse myelomas and lymphomas in culture. Exp. Cell Res. 60: 61-77, 1970. PubMed: 5439579

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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