91精品国产综合成人,国产精品午夜自在在线精品,国内精品久久久久影院老司,国内精品久久久久久久久野战,国产精品美女久久福利网站,亚洲国产一成人久久精品,国产精品久久久久久一区二区三区

熱門(mén)搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購(gòu)物車(chē) 1 種商品 - 共0元
當(dāng)前位置: 首頁(yè) > ATCC代理 > QT6
▍最近瀏覽歷史
▍更多產(chǎn)品
▍聯(lián)系我們
  • 0574-87157013
  • [email protected]
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號(hào)
  • 創(chuàng)e慧谷42號(hào)樓B幢401室
QT6
QT6
規(guī)格:
貨期:
編號(hào):B165547
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng) QT6
商品貨號(hào) B165547
Organism Coturnix coturnix japonica, quail, Japanese
Cell Type methylcholanthrene induced
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease fibrosarcoma
Applications
This cell line is a suitable transfection host.
Storage Conditions liquid nitrogen vapor phase
Derivation Derived from chemically induced tumors of Japanese quail.
Tumorigenic Yes, in animal host.
Effects Yes, in animal host and soft agar.
Virus Susceptibility Rous sarcoma virus
Avian encephalomyelitis virus
Rabies virus
Marek's disease virus
Complete Growth Medium Ham's F12K medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate supplemented with 10% tryptose phosphate broth and 5% bovine calf serum
Subculturing Resuspend cells by pipetting fresh medium across the monolayer. Cells may also be dislodged by hitting culture flask against the palm of the hand. Subculture at approximately 75% confluency. Use of 0.5% polyvinylpyrrolidone (PVP) in a trypsin-EDTA solution increases the viability when subculturing by enzymatic means. Neutralize and centrifuge to remove additive.
Note: When subcultures are prepared using pipetting or mechanical means the cells adhere lightly to the flask substrate and exhibit a tendency toward clumping. 
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Twice per week
Cryopreservation Complete growth medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37°C
Name of Depositor C Moscovici
Deposited As Coturnix coturnix japonica
References

Moscovici C, et al. Continuous tissue culture cell lines derived from chemically induced tumors of Japanese quail. Cell 11: 95-103, 1977. PubMed: 194709

Imagawa S, et al. Negative regulation of the erythropoietin gene expression by the GATA transcription factors. Blood 89: 1430-1439, 1997. PubMed: 9028967

Martin PT, Sanes JR. Integrins mediate adhesion to agrin and modulate agrin signaling. Development 124: 3909-3917, 1997. PubMed: 9367446

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
剑阁县| 永德县| 连平县| 伊吾县| 如皋市| 仲巴县| 利川市| 尼木县| 罗江县| 古蔺县| 太康县| 阳朔县| 托克托县| 依兰县| 富源县| 寿宁县| 沁水县| 五家渠市| 简阳市| 枣阳市| 灵寿县| 通城县| 达州市| 菏泽市| 滨州市| 特克斯县| 青海省| 太湖县| 波密县| 雅安市| 平安县| 蒙城县| 萝北县| 齐河县| 开封市| 新疆| 天全县| 上栗县| 含山县| 内丘县| 兴城市|