| 產(chǎn)品名稱 |
NMuMG |
| 商品貨號(hào) |
B165404 |
| Organism |
Mus musculus, mouse |
| Tissue |
mammary gland |
| Product Format |
frozen |
| Morphology |
epithelial |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Gender |
female |
| Strain |
NAMRU |
| Applications |
This cell line is a suitable transfection host |
| Clinical Data |
female |
| Tumorigenic |
Yes |
| Effects |
Yes, benign cystadenomas are produced in isogenic mice |
| Comments |
The cells do not express detectable retroviruses. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
Dulbecco's modified Eagle's medium with 4.5 g/L glucose and 10 mcg/ml insulin, 90%; fetal bovine serum, 10%
|
| Subculturing |
Remove medium, rinse with fresh 0.25% trypsin, 0.02% EDTA solution, remove trypsin and let the culture sit at room temperature (or at 37°C) until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week |
| Name of Depositor |
RB Owens |
| Deposited As |
Mus musculus |
| References |
Owens RB. Glandular epithelial cells from mice: a method for selective cultivation. J. Natl. Cancer Inst. 52: 1375-1378, 1974. PubMed: 4363715
Owens RB, et al. Epithelial cell cultures from normal glandular tissue of mice. J. Natl. Cancer Inst. 53: 261-269, 1974. PubMed: 4366196
Yingling JM, et al. Mammalian dwarfins are phosphorylated in response to transforming growth factor beta and are implicated in control of cell growth. Proc. Natl. Acad. Sci. USA 93: 8940-8944, 1996. PubMed: 8799132
|
