| 產(chǎn)品名稱 |
NF-1 |
| 商品貨號(hào) |
B165390 |
| Organism |
Mus musculus, mouse |
| Product Format |
frozen |
| Morphology |
epithelial |
| Culture Properties |
mixed adherent-suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
nullipotent embryonal carcinoma; teratocarcinoma |
| Age |
embryo; 7 days gestation |
| Gender |
male |
| Strain |
BALB/c |
| Applications |
This pluripotent stem cell line was established in 1979 by Fox et al. |
| Derivation |
This pluripotent stem cell line was established in 1979 by Fox et al. |
| Clinical Data |
When injected into the tail veins of female mice, the cells have a tendency to metastasize to the ovaries and adrenals. When injected into male mice, fewer, and slower growing, tumors are formed. male |
| Tumorigenic |
Yes |
| Effects |
Yes, when injected intramuscularly into syngeneic mice |
| Comments |
This pluripotent stem cell line was established in 1979 by Fox et al. When injected into the tail veins of female mice, the cells have a tendency to metastasize to the ovaries and adrenals. When injected into male mice, fewer, and slower growing, tumors are formed. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended This is a mixed cell culture; cells grow both as a monolayer and as a suspension. Subcultures are prepared by scraping the attached cell into the medium containing the floaters. Aspirate and dispense into new culture flasks.
Subculture approximately two times weekly. Cells will detach from surface of flask before confluency is reached.
|
| Cryopreservation |
Culture medium, 95%; DMSO, 5% |
| Culture Conditions |
Temperature: 37.0°C |
| Name of Depositor |
I Damjanov |
| Deposited As |
Mus musculus |
| Year of Origin |
1979 |
| References |
Fox N, et al. Male murine embryonal carcinoma cell line selectively metastatic to the ovaries and adrenals. Virchow's Arch. B Cell Pathol. 43: 241-251, 1983. PubMed: 6137898
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