91精品国产综合成人,国产精品午夜自在在线精品,国内精品久久久久影院老司,国内精品久久久久久久久野战,国产精品美女久久福利网站,亚洲国产一成人久久精品,国产精品久久久久久一区二区三区

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當前位置: 首頁 > ATCC代理 > NCI-BL2122 [BL2122]
▍最近瀏覽歷史
▍聯系我們
  • 0574-87157013
  • [email protected]
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號
  • 創(chuàng)e慧谷42號樓B幢401室
NCI-BL2122 [BL2122]
規(guī)格:
貨期:
編號:B165243
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產品名稱 NCI-BL2122 [BL2122]
商品貨號 B165243
Organism Homo sapiens, human
Tissue peripheral blood
Cell Type B lymphoblast; Epstein-Barr virus (EBV) transformed
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 2 [Cells contain Epstein-Barr Virus (Herpesvirus)]

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease Adenocarcinoma
Age 46 years
Gender female
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Derivation

NCI-H2122 was derived from pleural effusion from a 46 year old White female with adenocarcinoma of the lung.

NCI-BL2122 BL is a B lymphoblastoid cell line initiated by Epstein-Barr virus (EBV) transformation of peripheral blood lymphocytes obtained from the same patient as NCI-H2122.
Clinical Data
46 years
Caucasian
female
Comments
The tumor cell line from this patient is available as ATCC CRL-5985.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 to 7 x 105 viable cells/mL. A maximum of 1 to 2 x 106 cells/mL is obtainable. Cultures grow as floating aggregates of large cell clusters. Add medium as cell density increases. Double volume approximately every 6 to 7 days.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation
Culture medium, 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 10
D13S317: 9,10
D16S539: 9,12
D5S818: 11,12
D7S820: 8,10
THO1: 7,9.3
TPOX: 9
vWA: 17
Name of Depositor AF Gazdar, JD Minna
Deposited As Homo sapiens
References

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

梅經理 17280875617 1438578920
胡經理 13345964880 2438244627
周經理 17757487661 1296385441
于經理 18067160830 2088210172
沈經理 19548299266 2662369050
李經理 13626845108 972239479
利津县| 临西县| 凌海市| 铜梁县| 班玛县| 勃利县| 鹤庆县| 古交市| 方正县| 大渡口区| 岚皋县| 榆社县| 通城县| 黄陵县| 双辽市| 广水市| 新乐市| 道真| 蚌埠市| 中山市| 新安县| 土默特右旗| 怀集县| 肃南| 金沙县| 文水县| 太湖县| 天峻县| 包头市| 温宿县| 唐山市| 镇赉县| 英德市| 共和县| 石屏县| 婺源县| 诸城市| 郎溪县| 浑源县| 道真| 靖江市|