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MR1 [derivative of HB-11048]
MR1 [derivative of HB-11048]
規(guī)格:
貨期:
編號(hào):B165192
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 MR1 [derivative of HB-11048]
商品貨號(hào) B165192
Organism Cricetulus migratorius (B cell); Mus musculus (myeloma), hamster, Armenian (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Storage Conditions liquid nitrogen vapor phase
Derivation
Spleen cells were fused with NS-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against mouse CD40 ligand (CD154, CD40L, gp39)
Cellular Products
immunoglobulin; monoclonal antibody; against mouse CD40 ligand (CD154, CD40L, gp39)
Comments
Animals were immunized with mouse T cells (Th1).
Spleen cells were fused with NS-1 myeloma cells.
The antibody detects a 39000 dalton protein selectively expressed on activated mouse T cells.
A culture deposited at the ATCC as HB-11048 in May of 1992 was found to be contaminated with mycoplasma. Progeny were cured by a 21-day treatment with BM Cycline.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 10%
  • 0.05 mM 2-mercaptoethanol
Subculturing Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 x 105 viable cells/mL. Maintain cell density between 1 x 105 and 1 x 106 viable cells/mL.

Medium Renewal: Every 2 to 3 days
Cryopreservation

Complete growth medium described above supplemented with 5% (v/v) DMSO.  Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
Isotype IgG
Name of Depositor Bristol-Myers Squibb Pharm. Res. Inst.
Deposited As hamster (B cell); mouse (myeloma)
References

Noelle RJ, et al. A 39-kDa protein on activated helper T cells binds CD40 and transduces the signal for cognate activation of B cells. Proc. Natl. Acad. Sci. USA 89: 6550-6554, 1992. PubMed: 1378631

Roy M, et al. The regulation of the expression of gp39, the CD40 ligand, on normal and cloned CD4+ T cells. J. Immunol. 151: 2497-2510, 1993. PubMed: 8103067

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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