91精品国产综合成人,国产精品午夜自在在线精品,国内精品久久久久影院老司,国内精品久久久久久久久野战,国产精品美女久久福利网站,亚洲国产一成人久久精品,国产精品久久久久久一区二区三区

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當前位置: 首頁 > ATCC代理 > MC/9
▍最近瀏覽歷史
▍聯(lián)系我們
  • 0574-87157013
  • [email protected]
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號
  • 創(chuàng)e慧谷42號樓B幢401室
MC/9
MC/9
規(guī)格:
貨期:
編號:B165096
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 MC/9
商品貨號 B165096
Organism Mus musculus, mouse
Tissue liver
Cell Type mast cell
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Age 13 days
Strain (B6 X A/J)F1
Storage Conditions liquid nitrogen vapor phase
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
This is a cloned mast cell line derived from the fetal liver of a (B6 X A/J)F1 mouse.
Receptor Expression
FcERI (Fc of IgE)
Genes Expressed
histamine; leukotrienes
Cellular Products
histamine; leukotrienes
Comments
The cells can be sensitized to specific antigens by incubating them with IgE having the desired antigenic specificity.

The cells so coated will release soluble mediators (such as histamine) in response to exposure to the antigen (allergen).
Complete Growth Medium Dulbecco's Modified Eagle's Medium with 4 mM L-glutamine adjusted to contain 4.5 g/L glucose and 1.5 g/L sodium bicarbonate and supplemented with 2 mM L-glutamine, 0.05 mM 2-mercaptoethanol, 10% Rat T-STIM (Becton Dickenson Catalog No. 354115) and 10% fetal bovine serum.
Subculturing
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 to 5 X 105 viable cells/ml. Maintain cell density between 2 X 105 and 2 X 106 viable cells/mL. Do not allow the cell density to exceed 2 X 106 cells/mL.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Culture Conditions
Temperature: 37°C
Name of Depositor Dana Farber Cancer Inst.
Deposited As Mus musculus
U.S. Patent Number
References

Cantor HI, Nabel G. Assay methods and systems utilizing mast cell clones. US Patent 4,559,310 dated Dec 17 1985

Nabel G, et al. Use of cloned populations of mouse lymphocytes to analyze cellular differentiation. Cell 23: 19-28, 1981. PubMed: 6971154

Galli SJ, et al. Mast cell clones: a model for the analysis of cellular maturation. J. Cell Biol. 95: 435-444, 1982. PubMed: 6216259

Lewin I, et al. Aggregation of the FcepisilonRI in mast cells induces the synthesis of fos-interacting protein and increases its DNA binding activity: the dependence on protein kinase c-beta. J. Biol. Chem. 271: 1514-1519, 1996. PubMed: 8576146

Ishizuka T, et al. Aggregation of the FceRI on mast cells stimulates c-Jun amino-terminal kinase activity. J. Biol. Chem. 271: 12762-12766, 1996. PubMed: 8662803

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
巴里| 陆川县| 鹿邑县| 甘德县| 长沙市| 湟源县| 关岭| 清河县| 集贤县| 三亚市| 古蔺县| 宁津县| 塘沽区| 吉水县| 且末县| 京山县| 寿阳县| 凌源市| 长海县| 兖州市| 蒙阴县| 木里| 三原县| 巫溪县| 集贤县| 延边| 西华县| 新乡县| 惠水县| 宁都县| 虹口区| 梅州市| 栖霞市| 郓城县| 台北市| 高州市| 翁牛特旗| 祁东县| 呼玛县| 肥城市| 新安县|