91精品国产综合成人,国产精品午夜自在在线精品,国内精品久久久久影院老司,国内精品久久久久久久久野战,国产精品美女久久福利网站,亚洲国产一成人久久精品,国产精品久久久久久一区二区三区

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當前位置: 首頁 > ATCC代理 > H36.12j [Pixie 12j]
最近瀏覽歷史
聯(lián)系我們
  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號
  • 創(chuàng)e慧谷42號樓B幢401室
H36.12j [Pixie 12j]
H36.12j [Pixie 12j]
規(guī)格:
貨期:
編號:B164537
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產品名稱 H36.12j [Pixie 12j]
商品貨號 B164537
Organism Mus musculus (macrophage tumor cell line); Mus musculus (peritoneal macrophage), mouse(macrophage tumor cell line); mouse (peritoneal macrophage)
Cell Type Macrophage
Product Format frozen
Morphology macrophage
Culture Properties suspension (some adherent cells)
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
This cell line was established by P.A. Campbell and E.P. Canono in 1991. Percoll gradient purified, proteose peptone elicited peritoneal macrophage cells from C57BL/6N mice were fused with drug selected P388D1 mouse macrophage tumor cells.
The cells produce tumor necrosis factor alpha (TNF alpha) upon direct beryllium stimulation and serve as an in vitro model for chronic beryllium disease in humans.
Since intracellular Listeria escape into the cytoplasm where they replicate, the cell line is a useful tool in the study of the permissive intracellular growth cycle of Listeria.
These cells may be used in assays for the phagocytosis and killing of microorganisms, in assays for the production of cytokines by mouse macrophages, and for assays for the effect of environmental toxins on mouse macrophages.
Storage Conditions liquid nitrogen vapor phase
Derivation
This cell line was established by P.A. Campbell and E.P. Canono in 1991. Percoll gradient purified, proteose peptone elicited peritoneal macrophage cells from C57BL/6N mice were fused with drug selected P388D1 mouse macrophage tumor cells.
Genes Expressed
surface Interleukin-10 (Interleukin 10; IL-10),tumor necrosis factor alpha (TNF alpha) upon direct beryllium stimulation
Cellular Products
surface Interleukin-10 (Interleukin 10; IL-10)
tumor necrosis factor alpha (TNF alpha) upon direct beryllium stimulation
Comments
This cell line was established by P.A. Campbell and E.P. Canono in 1991. Percoll gradient purified, proteose peptone elicited peritoneal macrophage cells from C57BL/6N mice were fused with drug selected P388D1 mouse macrophage tumor cells.
H36.12j is a model for the permissive intracellular growth of Listeria monocytogenes within mouse macrophages. The cells phagocytose Listeria via receptors for internalin A (InlA), a Listeria surface polypeptide.
The cells produce tumor necrosis factor alpha (TNF alpha) upon direct beryllium stimulation and serve as an in vitro model for chronic beryllium disease in humans.
Since intracellular Listeria escape into the cytoplasm where they replicate, the cell line is a useful tool in the study of the permissive intracellular growth cycle of Listeria.
The H36.12j cells have been found to express biologically active surface IL-10 that regulates macrophage bacterial activity.
These cells may be used in assays for the phagocytosis and killing of microorganisms, in assays for the production of cytokines by mouse macrophages, and for assays for the effect of environmental toxins on mouse macrophages.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium:
  • heat-inactivated iron supplemented bovine calf serum to a final concentration of 10%
  • Subculturing Add fresh medium every 2 to 3 days (depending on cell density). Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 x 105 viable cells/mL. Maintain cell density between 1 x 105 and 1 x 106 viable cells/mL. Adherent cells may be harvested by scraping.

    Medium Renewal: Every 2 to 3 days
    Cryopreservation

    Complete growth medium described above supplemented with 5% (v/v) DMSO.  Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

    Culture Conditions
    Temperature: 37°C
    Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
    Name of Depositor RT Sawyer
    Deposited As mouse
    Year of Origin 1991
    References

    Canono EP, Campbell PA. Production of mouse inflammatory hybridomas. J. Tissue Culture Methods 14: 3-8, 1992.

    Fleming SD, Campbell PA. Macrophages have cell surface IL-10 that regulates macrophage bactericidal activity. J. Immunol. 56: 1143-1150, 1996. PubMed: 8557991

    Sawyer RT, et al. Internalin A can mediate phagocytosis of Listeria monocytogenes by mouse macrophage cell lines. J. Leukocyte Biol. 60: 603-610, 1996. PubMed: 8929551

    Fleming SD, Campbell PA. Some macrophages kill Listeria monocytogenes while others do not. Immunol. Rev. 158: 69-77, 1997. PubMed: 9314075

    Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

    Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

    Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

    梅經理 17280875617 1438578920
    胡經理 13345964880 2438244627
    周經理 17757487661 1296385441
    于經理 18067160830 2088210172
    沈經理 19548299266 2662369050
    李經理 13626845108 972239479
    宁晋县| 鱼台县| 乌兰察布市| 广德县| 安西县| 高安市| 蒙山县| 石林| 商水县| 启东市| 杂多县| 华容县| 旬阳县| 京山县| 太谷县| 焦作市| 沁水县| 万源市| 伊宁市| 石林| 呼玛县| 镇坪县| 迭部县| 屯昌县| 盐源县| 平塘县| 阳曲县| 玛多县| 永靖县| 庐江县| 桃源县| 余庆县| 舞阳县| 沁源县| 赤水市| 珲春市| 景宁| 莲花县| 西盟| 南丹县| 那曲县|