| 產(chǎn)品名稱 |
143-4-2 |
| 商品貨號(hào) |
B163572 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The line secretes an allele specific antibody that reacts with the Ly 6.2C mouse T cell surface antigen. The antibody is useful for studies of T cell activation and T cell surface antigen phenotypes. |
| Derivation |
Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against mouse Ly 6.2C |
| Cellular Products |
immunoglobulin; monoclonal antibody; against mouse Ly 6.2C |
| Comments |
Animals were immunized with the BM10-37 mouse cytotoxic T lymphocyte (CTL) cell clone (anti H-2 Kb). Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The line secretes an allele specific antibody that reacts with the Ly 6.2C mouse T cell surface antigen. The antibody promotes alloreactive CTL lysis of antigen non-bearing targets. It does not appear to interact directly with the T cell antigen receptor (TcR) as Ly 6.2C is not believed to be associated with the TcR complex on the T cell surface. The antibody is useful for studies of T cell activation and T cell surface antigen phenotypes. |
| Complete Growth Medium |
Iscove's modified Dulbecco's medium, 90%; fetal bovine serum, 10%
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype |
IgG1 |
| Name of Depositor |
JA Bluestone |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Leo O, et al. Activation of murine T lymphocytes with monoclonal antibodies: detection on Lyt-2+ cells of an antigen not associated with the T cell receptor complex but involved in T cell activation. J. Immunol. 139: 1214-1222, 1987. PubMed: 3497199
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