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YCpIF9
YCpIF9
規(guī)格:
貨期:
編號:B162363
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 YCpIF9
商品貨號 B162363
Designations YCpIF9
Depositors RW Davis, PK Foreman
Biosafety Level 1
Vector Information
Size (kb): 6.4000000953674320
Vector: YCpIF9 (plasmid)
Promoters: Promoter for expression GAL1
Construction: pUN95
Marker(s):ampR,HIS3
Construct size (kb): 6.400000095367432
Features: marker(s): HIS3
marker(s): ampR
promoter for expression: GAL1
replicon: ARS1
replicon: pMB1
MCS: SalI...SacI
centromere: CEN4
Applications
YX-type (expression) shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--4.0, 2.4; EcoRI--6.4; XbaI--6.4.
Care should be taken when cloning into this vector because several stop codons occur in this reading frame.
One of several S. cerevisiae shuttle expression vectors (ATCC 87078-87092) differing in selection marker, reading frame and orientation of the promoter.
Vectors YCpIF7, YCpIF8 and YCpIF9 (ATCC 87084-87086) differ only in the reading frame of the multiple cloning site.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Foreman PK, Davis RW. Cloning vectors for the synthesis of epitope-tagged, truncated and chimeric proteins in Saccharomyces cerevisiae. Gene 144: 63-68, 1994. PubMed: 7517907

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